DEFA5 antibody

Catalog No. ABIN396940

This Mouse Monoclonal antibody specifically detects DEFA5 in WB, IHC, IHC (p) and IF. It exhibits reactivity toward Human.

Quick Overview for DEFA5 antibody (ABIN396940)

Target: DEFA5 (Defensin, alpha 5, Paneth Cell-Specific (DEFA5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DEFA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-DEFA5 Antibody

Specificity: This antibody is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2. Species cross-reactivity: This antibody reacts with human HD5 protein. Other species have not been tested.

Purification: Purified

Immunogen: Full-length proHD5 protein. [Swiss-Prot# Q01523] The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine). Epitope: This antibody has not been epitope mapped.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IF, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-glycine, 150 mM sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for DEFA5

Target: DEFA5 (Defensin, alpha 5, Paneth Cell-Specific (DEFA5))

Alternative Name: DEFA5 / Defensin 5

Background: Name/Gene ID: DEFA5

Synonyms: DEFA5, DEF5, Defensin, alpha 5, Defensin 5, Defensin-5, HD-5, HD5(20-94)

Gene ID: 1670

UniProt: Q01523