B Tag antibody

Antigen: B Tag

Clonality: Polyclonal

Host: Rabbit

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN398470

Additional Information

Alternative name: B-tag

Format: Affinity-purified

Isotype: IgG  (Matching secondary antibodies)

Description: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The B-tag is an epitope tag composed of a six-residue peptide, QYPALT, corresponding to a highly conserved region of VP7 core protein of the blue tongue virus. The Antibodies-Online Rabbit Anti-B-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic B-tag peptide conjugated to KLH.Antibodies-Online Rabbit Anti-B-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.

Specificity: Antibodies-Online Rabbit Anti-B-tag Polyclonal Antibody recognizes either N-terminal or C-terminal B tagged fusion proteins.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/mlWestern blot: 0.1-1.0 ug/mlWestern blot using the ONE-HOUR WesternTM Kit: For quick results, the Antibodies-Online ONE-HOUR Western^TM Kit L00204C is recommended. 10 ug of this antibody is mixed with 10 ml of WB solution for an 8 cm X 8 cm membrane.Other applications: user-optimized

Concentration: 1 mg/ml in PBS, pH 7.4, containing 0.02% sodium azide

Purification: Immunoaffinity chromatography

Storage: Rabbit Anti-B-tag Polyclonal Antibody is stable for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Restrictions: For Research Use only

Images

Lane 1-2: B-tag fusion protein in E. coli cell lysate (~ 30 kD)Lane 3: Negative E. coli cell lysatePrimary antibody: 1ug/ml Rabbit Anti-B-tag Polyclonal Antibody (Antibodies-Online, ABIN398470)Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody (Antibodies-Online, ABIN398323, 1:10,000)The signal was developed with LumiSensorTM HRP Substrate Kit (Antibodies-Online, L00221V500)

Western blot analysis of B tagged fusion proteins expressed in E. coli cell lysate using Rabbit Anti-B-tag Polyclonal Antibody (Antibodies-Online, ABIN398470, 1 ug/ml)The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.