DDDDK Tag antibody

Catalog No. ABIN1573914

This Rabbit Polyclonal antibody specifically detects DDDDK Tag in WB and ELISA.

Quick Overview for DDDDK Tag antibody (ABIN1573914)

Target: DDDDK Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDDDK Tag Antibody

Sequence: DDDDK

Specificity: This Antibody recognizes the enterokinase cleavage site and specifically binds to fusion proteins containing C-terminal, N-terminal, and internal xxxDDDDK epitope tags, such as Xpress tag or Flag tag

Purification: Immunoaffinity chromatography

Immunogen: A synthetic epitope peptide xxxDDDDK conjugated - KLH. This sequence represents the enterokinase cleavage site (ECS).

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using the ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 5 µg of this antibody is mixed with 10 mL of WB solution for any 8 cm X 8 cm membrane.Other applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for DDDDK Tag

Target: DDDDK Tag

Alternative Name: DDDDK-Tag

Target Type: Tag

Background: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The xxxDDDDK-tag is a small size and high hydrophilicity epitope tag containing an enterokinase cleavage site that allows it to be completely removed from the fusion proteins.The Rabbit Anti-DDDDK-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic peptide containing xxxDDDDK epitope conjugated to KLH.This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml.