Donkey anti-Rabbit IgG Antibody (HRP)

Catalog No. ABIN400179

Quick Overview for Donkey anti-Rabbit IgG Antibody (HRP) (ABIN400179)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunoassay (IA)

Product Details

Reactivity: Rabbit

Host: Donkey

Purpose: Donkey anti-Rabbit IgG (H&L) - Affinity Pure, HRP Conjugate, min x w/mouse IgG or serum proteins

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on rabbit IgG • light chains on all rabbit immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • mouse IgG or serum proteins

Purification: Affinity purified using solid phase Rabbit IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Rabbit IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry 1:10,000-1:50,000 for ELISA/Western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: PBS, 1 % BSA & 0.1 % proclin 150, contains 0.1 % (v/v) Proclin 150

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Store lyophilized material at 2-8 °C. For long term storage after reconstitution, dilute with 50 % glycerol and store at -20 °C as a liquid.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Target Details

Target: IgG

Target Type: Antibody