Laminin A4 Chain antibody

Antigen: Laminin A4 Chain

Clonality: Monoclonal (9B2)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN400459

Additional Information

Characteristics: Monocloanl antibody to human laminin a4 chain

Immunogen: Immunoaffinity-purified Laminin 411 (laminin-8 (α4β1γ1) from Platelets.

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9B2

Description: Laminins are a family of large heterotrimeric proteins that promote cell adhesion and migration via integrins and other cell-surface receptors. They are major components of basement membranes of blood vessels and other tissue compartments, and are synthesized by numerous cell types. Through combination, laminin chains constitute 15 laminin isoforms. Laminin isoforms, particularly their α chain, are expressed in a cell- and tissue-specific manner, and are differentially recognized by integrins.

Application Details

Application Notes: ELISA: 1/8 000 Western immunoblotting, reduced conditions: 1/1 000 - 1/2 000 Immunoprecipitation: 1/20

Concentration: 1.00 mg/ml

Purification: IgG fraction, purified by ion exchange chromatography

Buffer: PBS pH 7.4, with 0.1% sodium azide

Storage: Store at -20 to -70 ˚C upon receipt. Avoid multiple freeze-thaw cycles as product degradation may result.

Research Area: Extracellular Matrix

Images

Reactivity of laminin alpha4 chain specific monoclonal antibody 9B8 on human platelet lysate by Western blotting (reducing, R and nonreducing, NR conditions).

Publications

Publications: Wondimu, Geberhiwot, Ingerpuu et al.: "An endothelial laminin isoform, laminin 8 (alpha4beta1gamma1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis." in: Blood, Vol. 104, Issue 6, pp. 1859-66, 2004 (PubMed).