COL18A1 antibody (Chain alpha 1)

Catalog No. ABIN400737

The Mouse Monoclonal anti-COL18A1 antibody (Clone 1873-46) (ABIN400737) specifically detects COL18A1 in WB and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for COL18A1 antibody (Chain alpha 1) (ABIN400737)

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This COL18A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1873-46

Product Details anti-COL18A1 Antibody

Binding Specificity: Chain alpha 1

Specificity: Recognizes Mouse Endostatin (Mr 22 kDa), an unidentified protein is also weakly detected (Mr 52 kDa).

Cross-Reactivity (Details): Species reactivity (expected):Human and Rat.
Species reactivity (tested):Mouse.

Characteristics: Synonyms: COL18A1, Endostatin, KNO1

Purification: Thiophilic Adsorption Chromatography.

Immunogen: Full-length Recombinant Murine Endostatin.

Isotype: IgG

Application Details

Application Notes: Western blot (Immunoblot). Immunohistochemistry on Paraffin Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 mM Sodium Phosphate, pH 8.0 containing 0.05 % Sodium Azide, before the addition of Glycerol to 30 %.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Background: Endostatin (20-22 kDa), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.Synonyms: COL18A1, Endostatin, KNO1

Gene ID: 80781

UniProt: P39060