MHV NSP9 antibody

Catalog No. ABIN400814

The Mouse Monoclonal anti-MHV NSP9 antibody is suitable to detect MHV NSP9 in samples from Mouse. It has been validated for WB and IF.

Quick Overview for MHV NSP9 antibody (ABIN400814)

Target: MHV NSP9

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 2C6-H1

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Characteristics: Synonyms: Nonstructural Protein 9, Mouse Hepatitis Virus Strain A59, Murine coronavirus

Purification: Protein A chromatography

Immunogen: E. coli derived fulllength MHV-A59 nsp9 protein. Remarks: This protein is part of the viral replicase polyprotein.

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1: 1,000 (Expect a band approximately 13 kDa in size corresponding to matureMHV-A59 nsp9 in the appropriate cell lysate or extract). Immunoflourescence: 1: 1,000 (Vero-E6 cells were grown on glass slides followed byinfection with MHVA59 strain and fixation with PBS/3 % PFA. After washing andpermeabilization of the fixed cells, antibody incubation was performed in PBS/5 %FCS for30 min). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C or below for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: MHV NSP9

Target Type: Viral Protein

Background: The nonstructural protein 9 (nsp9) is one of the Mouse hepatitis virus replicase cleavage products, encoded by ORF1a. Nsp9 is an RNA-binding protein that is thought to be part of the viral replication complex, which is associated with intracellular membranes.Synonyms: Mouse Hepatitis Virus Strain A59, Murine coronavirus, Nonstructural Protein 9

UniProt: P0C6X9