L1/ORF2 antibody

Antigen: L1/ORF2

Clonality: Polyclonal

Host: Chicken

Reactivity: Chimpanzee

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401096

Additional Information

Characteristics: Synonyms: LINE-1

Alternative name: L1 / ORF2

Immunogen: Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptidescorrespond to regions within the endonuclease domain of L1/ORF2 protein

Cross-Reactivity: Chimpanzee, Human

Isotype: IgG  (Matching secondary antibodies)

Description: The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most commonopen reading frame in the human genome, present in various forms in many thousands ofcopies. This large family of proteins includes magnesium dependent endonucleases and alarge number of phosphatases involved in intracellular signaling. Both intact ORF1 andORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes anendonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2appears to be tightly regulated except in germ line tissues, embryonic tissues, and certaincancers including teratomas, testicular cancers, and leukemias. This antibody is intendedfor use in studying control of L1 retrotransposons.

Specificity: This antibody reacts to L1 / ORF2. Species: Human, Chimpanzee. Others not tested.

Application Details

Application Notes: ELISA: 1/10000. Western Blot: 1/2000 - 1/10000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: Ig Fraction

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide as preservative

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch

Restrictions: For Research Use only