R-Phycoerythrin antibody (Biotin)

Catalog No. ABIN401158

This Goat Polyclonal antibody specifically detects R-Phycoerythrin in WB, IHC (fro), DB and EIA.

Quick Overview for R-Phycoerythrin antibody (Biotin) (ABIN401158)

Target: R-Phycoerythrin (Phycoerythrin, R-)

Host: Goat

Clonality: Polyclonal

Conjugate: This R-Phycoerythrin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB), Enzyme Immunoassay (EIA)

Product Details anti-R-Phycoerythrin Antibody (Biotin)

Characteristics: Molar Ratio: 10-20 BAC molecules per Goat IgG molecule

Purification: Immunoaffinity Chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Highly purified R-Phycoerythrin from the seaweed gracila

Isotype: IgG

Application Details

Application Notes: Suitable for Immunoblotting (western or dot blot), ELISA as well as other Immunochemicalassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate intocomponents upon exposure to reducing or denaturing agents. Reaction with low molecularweight fragments is typically noted by western blot. Recommneded Dilutions: ELISA: 1/200,000. Western Blot: 1/2000-1/20,000. Immunohistochemistry: 1/500-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for R-Phycoerythrin

Target: R-Phycoerythrin (Phycoerythrin, R-)

Alternative Name: R-Phycoerythrin

Target Type: Chemical

Background: Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB

Gene ID: 2775

UniProt: Q7SIF9