Phycoerythrin, R- antibody

Antigen: Phycoerythrin, R-

Clonality: Polyclonal

Host: Goat

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunodiffusion (ID), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN401161

Additional Information

Characteristics: Synonyms: rpeB

Alternative name: R-Phycoerythrin

Swiss-Prot: Q7SIF9

Immunogen: Highly purified R-Phycoerythrin from the seaweed gracila

Isotype: IgG  (Matching secondary antibodies)

Description: Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, whichare produced by red and blue green algae. It occurs in more than one form, and has foundapplication in immunology and diagnostic medicine. B and R Phycoerythrins providesuperior labeling compared to fluorescein and rhodamine, and are used for labelingantibodies, usually monoclonals. These dyes may also be coupled to enzymes and otherproteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorblight maximally between 450 and 650nm they fill the need for an intense fluorescent dye inthe longer wavelengths of the visible spectrum, thereby avoiding interference fromnaturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile moleculethat may dissociate into components upon exposure to reducing or denaturing agents.

Specificity: This antibody reacts to R-Phycoerythrin. This antibody will cross react with B-Phycoerythrin. Reactivity with otherphycobiliproteins is unknown.

Application Details

Application Notes: Western Blot: 1/2000 - 1/10000. Dot Blot. ELISA: 1/20000 - 1/100000. Immunohistochemistry: 1/1000 - 1/5000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.2 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only