Transferrin antibody

Catalog No. ABIN401207

The Rabbit Polyclonal anti-Transferrin antibody is suitable to detect Transferrin in samples from Mouse. It has been validated for WB, EIA and IHC (fro).

Quick Overview for Transferrin antibody (ABIN401207)

Target: Transferrin (TF)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Transferrin Antibody

Specificity: This antibody detects Transferrin.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The eluate was dialyzed against PBS with sodium azide, concentrated by Amicon ultrafiltration, 0.2 µm filtered, aseptically filled, and stored at -20° C. An aliquot was submitted for final qualification.

Immunogen: Mouse Transferrin

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Target Details for Transferrin

Target: Transferrin (TF)

Background: Synonyms: Beta-1 metal-binding globulin, Serotransferrin, Siderophilin

Gene ID: 22041

NCBI Accession: NP_598738

UniProt: Q921I1

Pathways: Transition Metal Ion Homeostasis