PACRG antibody (AA 204-215)

Catalog No. ABIN401250

The Rabbit Polyclonal anti-PACRG antibody (ABIN401250) specifically detects PACRG in WB and EIA. The antibody is reactive with Human, Mouse and Chicken samples.

Quick Overview for PACRG antibody (AA 204-215) (ABIN401250)

Target: PACRG (PARK2 Co-Regulated (PACRG))

Reactivity: Human, Mouse, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PACRG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PACRG Antibody

Binding Specificity: AA 204-215

Specificity: This antibody is directed against human PACRG protein. Expect reactivity with both splice variants of this protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and chicken (100 %).Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94 % homology).
Species reactivity (tested):Human.

Purification: Affinity chromstography

Immunogen: Synthetic peptide corresponding to amino acids 204-215 of Human PACRG protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1: 2,000 - 1: 10,000. Western blot: 1: 500 - 1: 3,000 (Expect a band approx. 40 kDa in size corresponding to PACRG inthe appropriate cell lysate or extract).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.

Target Details for PACRG

Target: PACRG (PARK2 Co-Regulated (PACRG))

Alternative Name: PACRG

Background: PACRG (also known as Parkin coregulated gene protein and PARK2 coregulated) is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be cotranscribed with parkin by a bi-directional promoter between the two genes. PACRG is expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow and is also expressed in heart, brain, skeletal muscle, kidney, lung and pancreas. PACRG is expressed in primary Schwann cells and very weakly by monocyte-derived macrophages, which are the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Splice variants have been described for this protein.Synonyms: GLUP, Molecular chaperone/chaperonin-binding protein, PARK2 coregulated gene protein, PARK2CRG, Parkin coregulated gene protein

Gene ID: 135138

NCBI Accession: NP_001073847

UniProt: Q96M98