APC1 antibody (pSer355)

Catalog No. ABIN401294

The Rabbit Polyclonal anti-APC1 antibody has been validated for WB and EIA. It is suitable to detect APC1 in samples from Human, Mouse, Rat, Cow, Dog, Chicken and Chimpanzee.

Quick Overview for APC1 antibody (pSer355) (ABIN401294)

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Chicken, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-APC1 Antibody

Binding Specificity: AA 351-359, pSer355

Specificity: This antibody is specific for phosphorylated APC1 protein at the pS355 residue.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Chimpanzee, Chicken.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 351-359 of Human Apc1 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.55 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for APC1

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Alternative Name: APC1 / ANAPC1

Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.Synonyms: Anaphase-promoting complex subunit 1, Cyclosome subunit 1, MCPR, Mitotic checkpoint regulator, TSG24, Testis-specific gene 24 protein

Gene ID: 64682

NCBI Accession: NP_073153

UniProt: Q9H1A4