LacI antibody

Catalog No. ABIN401452

This Rabbit Polyclonal antibody specifically detects LacI in WB and EIA. It exhibits reactivity toward E. coli and Shigella sonnei.

Quick Overview for LacI antibody (ABIN401452)

Target: LacI

Reactivity: E. coli, Shigella sonnei

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details

Specificity: This antibody reacts with recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100 % homology with the immunizing sequence.

Purification: Affinity Chromatography

Immunogen: Full-length recombinant Lac I protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/90,000-1/100,000. Western Blot: 1/500-1/2,000. Expect a band approximately approx. 38 kDa in size corresponding to Lac I by Western blotting inthe appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details

Target: LacI

Background: The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase, modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein. This antibody could possibly be used to recover and purify protein/nucleic acid complexes (host and viral proteins associated with the viral DNA) from infected cells.Synonyms: JW0336, Lactose operon repressor, b0345

Gene ID: 945007

NCBI Accession: AP_000997

UniProt: P03023