PRDM1 antibody (C-Term)

Catalog No. ABIN401477

The Rabbit Polyclonal anti-PRDM1 antibody is suitable to detect PRDM1 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for PRDM1 antibody (C-Term) (ABIN401477)

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRDM1 Antibody

Binding Specificity: C-Term

Specificity: This antibody is directed against BLIMP1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the C-terminus of human, mouse, and rat PRDM1/BLIMP1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/65,000 - 1/80,000. Western Blot: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.15 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for PRDM1

Target: PRDM1 (PR Domain Containing 1, with ZNF Domain (PRDM1))

Alternative Name: PRDM1 / BLIMP1

Background: PRDM1/Blimp1 (PR domain zinc finger protein 1, PR domain-containing protein 1, Beta-interferon gene positive regulatory domain I-binding factor, BLIMP-1, Positive regulatory domain I-binding factor 1, PRDIbinding factor 1, or PRDI-BF1, or Betalymphocyte-induced maturation protein 1) is one of several transcription factors which drives the maturation of B-lymphocytes into Ig secreting cells. In the nucleus, PRDM1/BLIMP1 functions as a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Transcription of the PRDM1 gene is increased upon virus induction. PRDM1/Blimp1 also interacts with PRMT5. Two alternatively spliced transcript variants that encode different isoforms have been reported.Synonyms: BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain zinc finger protein 1, PR domain-containing protein 1, PRDI-BF1, PRDI-binding factor 1, Positive regulatory domain I-binding factor 1

Gene ID: 639

NCBI Accession: NP_001189

UniProt: O75626

Pathways: Regulation of Muscle Cell Differentiation