CNN2 antibody (Internal Region)

Catalog No. ABIN401514

The Goat Polyclonal anti-CNN2 antibody (ABIN401514) specifically detects CNN2 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for CNN2 antibody (Internal Region) (ABIN401514)

Target: CNN2 (Calponin 2 (CNN2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CNN2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CNN2 Antibody

Binding Specificity: Internal Region

Sequence: c-DPGEVPEYPP YYQEE

Specificity: This antibody detects Calponin-2 (Internal). It is expected to recognize both reported isoforms (NP_004359.1, NP_958434.1), but it is not expected to cross-react with CCN3.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near C Terminus) of the protein sequence according to NP_004359.1, NP_958434.1. Genename: CNN2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: Recommended concentration: 0.03-0.1 μg/mL. Approx 35 kDa band observedin lysates of cell line HepG2 (calculated MW of 33.5 kDa according to NP_004359.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CNN2

Target: CNN2 (Calponin 2 (CNN2))

Alternative Name: Calponin-2

Background: The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: CNN2, Calponin H2, Neutral calponin

Gene ID: 1265

NCBI Accession: NP_004359

UniProt: Q99439