E2F7 antibody (Internal Region)

Catalog No. ABIN401533

This Goat Polyclonal antibody specifically detects E2F7 in WB and EIA. It exhibits reactivity toward Human, Dog and Pig.

Quick Overview for E2F7 antibody (Internal Region) (ABIN401533)

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Reactivity: Human, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This E2F7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-E2F7 Antibody

Binding Specificity: Internal Region

Sequence: c-DLSKQKKFTP ERNP

Specificity: This antibody detects E2F7 Transcription Factor (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_976328.2 . Genename: E2F7

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx 100 kDa band observed in Human Brain (Cerebellum),Kidney and Testis lysates (calculated MW of 99.0 kDa according to NP_976328.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer

Target Details for E2F7

Target: E2F7 (E2F Transcription Factor 7 (E2F7))

Alternative Name: E2F7

Background: E2F7 is an inhibitor of E2F-dependent transcription. It regulates a subset of E2F-dependent genes whose products are required for normal cell cycle progession as well as mitigate cellular proliferation of mouse embryo fibroblasts.Synonyms: E2F transcription factor 7, E2F-7, FLJ12981, Transcription factor E2F7

Gene ID: 144455

NCBI Accession: NP_976328

UniProt: Q96AV8