TNKS antibody (N-Term)

Catalog No. ABIN401535

Product Details anti-TNKS Antibody

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNKS antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects Tankyrase-1 (TANK1) at N-term. No cross reactivity expected to TANK2.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by affinity chromatography using Protein G

Immunogen: Fusion protein containing 120aa amino acids from N Terminal region of the protein sequence according to NP_003738.2 . Genename: TNKS

Application Details

Application Notes: Western blot: In transfected HEK293 transiently expressing TANK1 a band of approx.142 kDa is observed. This band is not observed in the non-transfected HEK293. Thecalculated molecular size is 142 kDa according to NP_003738.2. Recommendedconcentration, 0.5-1.5 μg/mL. Full caption )see picture below): HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4)and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for TNKS

Target: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Alternative Name: Tankyrase-1 (TANK1) (TNKS Products)

Synonyms: wu:fe02c12 antibody, ARTD5 antibody, PARP-5a antibody, PARP5A antibody, PARPL antibody, TIN1 antibody, TINF1 antibody, TNKS1 antibody, pART5 antibody, 4930554K12Rik antibody, AI662855 antibody, C86528 antibody, D130072O21Rik antibody, TANK1 antibody, mTNKS1 antibody, tankyrase-1 antibody, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b antibody, tankyrase antibody, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase antibody, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 S homeolog antibody, tnksb antibody, TNKS antibody, Tnks antibody, tnks2.S antibody

Background: Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate ribose) polymerase (PARP). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats. TRF1 is a negative regulator of telomere length maintenance. Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP) ribosylation. Since ADP ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP) ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. During interphase tankyrase colocalizes with TRF1 to telomeres plus nuclear pore complexes whereas during mitosis tankyrase localizes around the pericentriolar matrix of mitotic chromosomes. Tankyrase may regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4 vesicles.Synonyms: PARP5A, PARPL, TIN1, TINF1, TNKS, TNKS-1, TNKS1, TRF1-interacting ankyrin-related ADP-ribose polymerase, Tankyrase I

Gene ID: 8658

NCBI Accession: NP_003738

UniProt: O95271