ATP2B3 antibody (N-Term)

Catalog No. ABIN405647

The Rabbit Polyclonal anti-ATP2B3 antibody has been validated for WB. It is suitable to detect ATP2B3 in samples from Human, Rat, Mouse, Cow, Guinea Pig, Horse, Dog, Rabbit and Pig.

Quick Overview for ATP2B3 antibody (N-Term) (ABIN405647)

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Reactivity: Human, Rat, Mouse, Cow, Guinea Pig, Horse, Dog, Rabbit, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP2B3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP2B3 Antibody

Binding Specificity: N-Term

Sequence: GDMANSSIEF HPKPQQQRDV PQAGGFGCTL AELRTLMELR GAEALQKIEE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 79%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ATP2B3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP2B3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1220 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP2B3

Target: ATP2B3 (ATPase, Ca++ Transporting, Plasma Membrane 3 (ATP2B3))

Alternative Name: ATP2B3

Background: ATP2B3 gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. ATP2B3 is the plasma membrane calcium ATPase isoform 3.The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 3. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: PMCA3, PMCA3a
Protein Interaction Partner: LGR4, PARK2, UBC, TERF2,
Protein Size: 1220

Molecular Weight: 134 kDa

Gene ID: 492

NCBI Accession: NM_001001344, NP_001001344

UniProt: Q16720

Pathways: Ribonucleoside Biosynthetic Process