GCFC2 antibody (N-Term)

Catalog No. ABIN406658

The Rabbit Polyclonal anti-GCFC2 antibody has been validated for WB. It is suitable to detect GCFC2 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse and Rabbit.

Quick Overview for GCFC2 antibody (N-Term) (ABIN406658)

Target: GCFC2 (GC-Rich Sequence DNA-Binding Factor 2 (GCFC2))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCFC2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GCFC2 Antibody

Binding Specificity: N-Term

Sequence: SEPDDHEKRI PFTLRPQTLR QRMAEESISR NEETSEESQE DEKQDTWEQQ

Predicted Reactivity: Cow: 79%, Dog: 100%, Guinea Pig: 92%, Horse: 85%, Human: 100%, Mouse: 85%, Rabbit: 83%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against C2orf3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human C2orf3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 781 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GCFC2

Target: GCFC2 (GC-Rich Sequence DNA-Binding Factor 2 (GCFC2))

Alternative Name: C2orf3

Background: The first mRNA transcript isolated for C2orf3 gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). C2orf3 belongs to the GCF family. It is a factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters.The first mRNA transcript isolated for this gene was part of an artificial chimera derived from two distinct gene transcripts and a primer used in the cloning process (see Genbank accession M29204). A positively charged amino terminus present only in the chimera was determined to bind GC-rich DNA, thus mistakenly thought to identify a transcription factor gene.
Alias Symbols: DNABF, GCF, TCF9, C2orf3
Protein Interaction Partner: HDAC11, CREB3L2, ATXN1, SUMO2,
Protein Size: 781

Molecular Weight: 89 kDa

Gene ID: 6936

NCBI Accession: NM_003203, NP_003194

UniProt: P16383