ATP6V1C1 antibody (N-Term)

Catalog No. ABIN406715

There is 1 publication for this product available. The Rabbit Polyclonal anti-ATP6V1C1 antibody is suitable to detect ATP6V1C1 in samples from Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for ATP6V1C1 antibody (N-Term) (ABIN406715)

Target: ATP6V1C1 (ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C1 (ATP6V1C1))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1C1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1C1 Antibody

Binding Specificity: N-Term

Purpose: ATP6V1C1 Antibody - N-terminal region

Sequence: ldafvegvvk kvaqymadvl edskdkvqen llangvdlvt yitrfqwdma

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ATP6V1C1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V1C1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58589-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATP6V1C1 antibody (ABIN406715)

Meo-Evoli, Almacellas, Massucci, Gentilella, Ambrosio, Kozma, Thomas, Tauler: "V-ATPase: a master effector of E2F1-mediated lysosomal trafficking, mTORC1 activation and autophagy." in: Oncotarget, Vol. 6, Issue 29, pp. 28057-70, (2015) (PubMed).

Target Details for ATP6V1C1

Target: ATP6V1C1 (ATPase, H+ Transporting, Lysosomal 42kDa, V1 Subunit C1 (ATP6V1C1))

Alternative Name: ATP6V1C1

Background:

Targeted Subunit: C 1

Background Information: ATP6V1C1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: ATPase, H+ transporting, lysosomal 42 kDa, V1 subunit C1

Alternative Symbols: VATC, Vma5, ATP6C, ATP6D

Protein Name: V-type proton ATPase subunit C 1

Molecular Weight: 42kDa

Gene ID: 528

NCBI Accession: NP_001686

UniProt: P21283

Pathways: Transition Metal Ion Homeostasis, Proton Transport