POU Class 5 Homeobox 1 (POU5F1) (transcript variant 1) antibody

Antigen: POU Class 5 Homeobox 1 (POU5F1)

Synonyms: OCT3, OCT4, OTF3, OTF4, MGC22487, POU5F1, POU5F1L

Epitope: transcript variant 1

Clonality: Monoclonal (9B7)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN410936

Additional Information

Alternative name: POU class 5 homeobox 1 (POU5F1), transcript variant 1

Gene ID: 5460

Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-360 of human OCT4

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9B7

Molecular Weight: 38.4 kDa

Application Details

Application Notes: Western blot: 1:5000, IF (1:100)

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Restrictions: For Research Use only

Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OCT-4(RC211998) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OCT-4. Oct4 antibody (9B7) at 1:5000 dilution + lysates from HEK-293T cell transfected with human OCT4 expression vector

anti-POU Class 5 Homeobox 1 (POU5F1) (transcript variant 1) antibody (Image 2)

Anti-OCT-4 mouse monoclonal antibody (ABIN410936) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OCT-4(RC211998).