Fumarate Hydratase, Nuclear Gene Encoding Mitochondrial Protein antibody

Antigen: Fumarate Hydratase, Nuclear Gene Encoding Mitochondrial Protein

Clonality: Monoclonal (9G4)

Host: Mouse

Reactivity: Human, Dog (Canine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN410988

Additional Information

Alternative name: fumarate hydratase (FH), nuclear gene encoding mitochondrial protein

Gene ID: 2271

Immunogen: Protein expressed in 293T cell transfected with human FH expression vector

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 9G4

Molecular Weight: 54.6 KDa

Application Details

Application Notes: WB 1:1000, IHC 1:50,

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Guaranteed Application * WB, IHC Suggested Dilutions WB 1:1000, IHC 1:50,

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Research Area: Cancer, Metabolism

Restrictions: For Research Use only

Images

Immunohistochemical staining of paraffin-embedded breast tissue using anti-FH mouse monoclonal antibody. (ABIN410988, Dilution 1:50) Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-FHmouse monoclonal antibody. (ABIN410988, Dilution 1:50)

anti-Fumarate Hydratase, Nuclear Gene Encoding Mitochondrial Protein antibody (Image 2)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH. Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FH monoclonal antibody. IHC Image Immunohistochemical staining of paraffin-embedded breast tissue using anti-FH mouse monoclonal antibody. (ABIN410988, Dilution 1:50)