Interferon gamma antibody

Catalog No. ABIN452485

This Mouse Monoclonal antibody specifically detects Interferon gamma in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Interferon gamma antibody (ABIN452485)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 165

Product Details anti-Interferon gamma Antibody

Specificity: This antibody recognizes both, recombinant and native human IFN-gamma.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: IFN-gamma, gamma IFN

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified recombinant Human Interferon gamma (IFN gamma).

Isotype: IgG1

Application Details

Application Notes: ELISA: Reacts with IFN-gamma at 0.1 ng/mL dilution of monoclonal antibody. Western Blot: IFN gamma antibody (ABIN452485) used at a concentration of 0.5 μg/mL,will allow visualization of 100 ng/lane of Interferon gamma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with double distillated water is recommended and to adjust the final concentration to 1.00 mg/mL

Concentration: 1.0 mg/mL

Buffer: 0.01 M PBS, pH 7.0 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFNG / Interferon gamma

Background: Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.Synonyms: IFN-gamma, gamma IFN

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy