Helicobacter Pylori antibody

Catalog No. ABIN452543

The Mouse Monoclonal anti-Helicobacter Pylori antibody is suitable to detect Helicobacter Pylori in samples from Helicobacter pylori. It has been validated for WB, EIA and ELISA (Capture).

Quick Overview for Helicobacter Pylori antibody (ABIN452543)

Target: Helicobacter Pylori (H. pylori)

Reactivity: Helicobacter pylori

Host: Mouse

Clonality: Monoclonal

Conjugate: This Helicobacter Pylori antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: 1-00E-010

Product Details anti-Helicobacter Pylori Antibody

Characteristics: Synonyms: H. pylori CagA Gene Product, Helicobacter pylori cytokine associated gene A

Purification: Affinity Chromatography on Protein G.

Immunogen: A highly immunogenic 32Kd CagA fragment, recombinant.

Isotype: IgG2a

Application Details

Application Notes: ELISA: HP CagA antibody (Clone 10E9) can be used as Capture antibody. In Sandwich ELISA, anti-HP CagA antibody clone 5H10 (Cat#ABIN452544) coated wellsmatches with HRP conjugated clone 5C6, clone 3C10 and clone 3C1 to detect therecombinant antigen. In addition, this clone selectively detected cell lysate of a CagA containing HP stain whenHRP conjugated clone 3C10 was used. Western Blotting: This clone detected a 32 KD band corresponding to the molecular weightof the recombinant CagA antigen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.0 without preservatives

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Helicobacter Pylori

Target: Helicobacter Pylori (H. pylori)

Alternative Name: Helicobacter Pylori

Target Type: Virus

Background: Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20 % may develop gastritis and gastric ulcer and 1-2 % may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.Synonyms: H. pylori