Heat Shock Factor 2 (HSF2) antibody

Antigen: Heat Shock Factor 2 (HSF2)

Synonyms: MGC75048, MGC117376, MGC156196, AI661205, zfHSF2, hsf2

Clonality: Monoclonal (3E02)

Host: Rat

Reactivity: Various Species

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Electrophoretic Mobility-Shift Assay (EMSA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN452671

Additional Information

Characteristics: Synonyms: HSF-2, HSTF2, Heat shock transcription factor 2

Alternative name: Heat shock factor 2 / HSF2

Swiss-Prot: P38533

Immunogen: Purified recombinant mouse HSF2 protein

Cross-Reactivity: Various Species

Isotype: IgG1  (Matching secondary antibodies)

Clone: 3E02

Description: HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors thatactivate the transcription of genes encoding products required for protein folding,processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shockproteins) expression by stressors is achieved at the level of transcription through a heatshock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNAbinding domain at the Nterminus. Hydrophobic repeats located adjacent to this bindingdomain are essential for the formation of active trimers. Towards the C-terminal regionanother short hydrophobic repeat exists, and is thought to be necessary for suppression oftrimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms,however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nucleardistribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes todiscrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates fromthese granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar tomouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally activethan the smaller beta form (7, 8). Various experiments have suggested that HFS2 may haveroles in differentiation and development (9, 10, 11).

Specificity: This antibody detects an ~ 69kDa protein in unstressed cells corresponding to HSF2 onSDS-PAGE immunoblots. Species Reactivity: Tested: Human, Mouse, Rat, Guinea Pig, Hamster, Monkey, Rabbit, Dog, Bovine, Sheep,Pig

Application Details

Application Notes: Western blot: 4 µg/ml was sufficient for detection of HSF2 of HeLa lysate. Gel shift assay (EMSA). Immunoprecipitation. Immunflourescence. Immunohistochemistry on frozen sections. ELISA.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, pH 7.2, 0.09% sodium azide in 50% glycerol

Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only