phosphoprotein Enriched in Astrocytes 15 (PEA15) (C-Term) antibody

Details for Product No. ABIN453779
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Antigen
Synonyms PEA15, DKFZp459O1410, HMAT1, HUMMAT1H, MAT1, MAT1H, PEA-15, PED, Pea15a, Mat1, Pea15, Pkcs15
Epitope
C-Term
(25), (20), (12), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(99), (72), (67), (36), (25), (3), (1)
Host
Rabbit
(98), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
ELISA, Western Blotting (WB)
(56), (48), (33), (30), (14), (13), (3), (1), (1)
Pubmed 2 references available
Quantity 0.4 mL
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Catalog No. ABIN453779
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Immunogen KLH conjugated synthetic peptide selected from the C-terminal region of human PEA-15
Specificity This antibody reacts to PEA15.
Purification Affinity chromatography on Protein A
Alternative Name PEA15
Background PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.
Alternate names: Astrocytic phosphoprotein PEA-15, PED, Phosphoprotein enriched in diabetes
Gene ID 8682
NCBI Accession NP_003759
UniProt Q15121
Research Area Apoptosis/Necrosis
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09% (w/v) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Trencia, Perfetti, Cassese et al.: "Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4511-21, 2003 (PubMed).

Sugiyama, Masuda, Shinoda et al.: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Validation Images
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