Afamin antibody (Internal Region)

Catalog No. ABIN453974

The Goat Polyclonal anti-Afamin antibody is suitable to detect Afamin in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for Afamin antibody (Internal Region) (ABIN453974)

Target: Afamin (AFM)

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Afamin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Afamin Antibody

Binding Specificity: Internal Region

Specificity: Recognizes Afamin / AFM.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NKDDRPKDLSLRE, from the internal region of Human AFM/Afamin according to NP_001124.1.

Application Details

Application Notes: Peptide ELISA: Afamin Antibody Detection limit dilution: 1/16000. Western blot: 0.3-1.0 μg/mL. Approx 80 kDa band observed in Human Brain (Cerebellumand Cerebral Cortex) lysates (calculated MW of 69.0 kDa according to NP_001124.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Afamin

Target: Afamin (AFM)

Alternative Name: Afamin / AFM

Background: AFM (afamin) is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These genes encode structurally related serum transport proteins that are thought to be evolutionarily related. AFM is regulated developmentally, expressed in the liver and secreted into the bloodstreamSynonyms: ALB2, ALBA, Alpha-albumin

Gene ID: 173

NCBI Accession: NP_001124

UniProt: P43652