TLN1 antibody (Internal Region)

Catalog No. ABIN453977

The Goat Polyclonal anti-TLN1 antibody is suitable to detect TLN1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB and EIA.

Quick Overview for TLN1 antibody (Internal Region) (ABIN453977)

Target: TLN1 (Talin 1 (TLN1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TLN1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TLN1 Antibody

Binding Specificity: Internal Region

Specificity: Recognizes Talin-1 (TLN1). This antibody is not expected to cross-react with the highly similar protein Talin 2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QEALHTQMLTAVQE, from the internal region of Human Talin 1 (NP_006280.3.)

Application Details

Application Notes: Peptide ELISA: Talin 1antibody Detection limit dilution: 1/8000. Western blot: 1-3 μg/mL. A >250 kDa band observed in Human Peripheral Mononucleocytelysates (calculated MW of 270 kDa according to NP_006280.3). An additional band of unknown identity was also consistently observed at 110 kDa. Thisband was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TLN1

Target: TLN1 (Talin 1 (TLN1))

Alternative Name: Talin-1 (TLN1)

Background: Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-270 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations.Synonyms: KIAA1027, TLN

Gene ID: 7094

NCBI Accession: NP_006280

UniProt: Q9Y490

Pathways: Cell-Cell Junction Organization, ER-Nucleus Signaling, Maintenance of Protein Location