CILP antibody (Internal Region)

Catalog No. ABIN453982

This Goat Polyclonal antibody specifically detects CILP in WB and EIA. It exhibits reactivity toward Human, Rat, Mouse and Dog.

Quick Overview for CILP antibody (Internal Region) (ABIN453982)

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This CILP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CILP Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes CILP.

Cross-Reactivity (Details): Species reactivity (expected):Human, Mouse and Canine (Dog).
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NYRRTDHEDPRVK, from the internal region of the protein sequence according to NP_003604.2.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/128000. Western Blot: 0.1-0.3 μg/mL. Approx 150 kDa band observed in rat spinal cord lysates(calculated MW of 130 kDa according to human NP_003604.2 and of 130 kDa according toRat NP_001101631.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CILP

Target: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Alternative Name: CILP1

Background: CILP, Cartilage Intermediate Layer Protein, is specifically expressed in the articular cartilage intermediate layer (it cannot be found in the superficial nor in the deepest layers) and is involved in scaffolding. Overexpression of this protein may lead to impairment of chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Antibodies against CILP are detected in patients with early-stage knee osteoarthritis and defects in this gene may be a cause of susceptibility to lumbar disk disease.Synonyms: CILP-1, Cartilage intermediate layer protein 1, Cartilage intermediate-layer protein

Gene ID: 8483

NCBI Accession: NP_003604

UniProt: O75339