Cell Adhesion Molecule 4 antibody (Internal Region)

Catalog No. ABIN453984

Cited in 1 publication. The Goat Polyclonal anti-Cell Adhesion Molecule 4 antibody (ABIN453984) specifically detects Cell Adhesion Molecule 4 in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cell Adhesion Molecule 4 antibody (Internal Region) (ABIN453984)

Target: Cell Adhesion Molecule 4 (CADM4)

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Cell Adhesion Molecule 4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Cell Adhesion Molecule 4 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes CADM4 (internal)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KDERFQLEEFS, from the internal region of the protein sequence according to NP_660339.1.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/16000. Western Blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of42.0 kDa according to NP_660339.1). The observed molecular weight corresponds tofindings with anti-CADM4 antibody (C-term) ABIN374902, a product of different designreacting to a different part of the same protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Cell Adhesion Molecule 4 antibody (ABIN453984)

Heffernan, Jain, Liu, Kim, Barretto, Li, Maurel: "Nectin-like 4 Complexes with Choline Transporter-like Protein-1 and Regulates Schwann Cell Choline Homeostasis and Lipid Biogenesis in Vitro." in: The Journal of biological chemistry, Vol. 292, Issue 11, pp. 4484-4498, (2017) (PubMed).

Target Details for Cell Adhesion Molecule 4

Target: Cell Adhesion Molecule 4 (CADM4)

Alternative Name: CADM4

Background: Cell Adhesion Molecule 4 (CADM4) is involved in the cell-cell adhesion. It has calcium and magnesium independent cell-cell adhesion activity and may have tumor suppressor activity.Synonyms: Cell adhesion molecule 4, IGSF4C, Immunoglobulin superfamily member 4C, NECL4, Nectin-like protein 4, TSLC1-like protein 2, TSLL2

Gene ID: 199731

NCBI Accession: NP_660339

UniProt: Q8NFZ8