Selectin L (SELL) antibody (FITC)

Antigen: Selectin L (SELL)

Synonyms: TQ1, LAM1, LNHR, LSEL, CD62L, LAM-1, LYAM1, Leu-8, PLNHR, hLHRc, LECAM1, Lyam-1, Lnhr, Ly-22, Lyam1, Ly-m22, LECAM-1, AI528707, L-selectin

Clonality: Monoclonal (DREG56)

Host: Mouse

Reactivity: Human

Conjugate: FITC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN457338

Additional Information

Alternative name: CD62L

Immunogen: PMA-activated human peripheral blood leukocytes

Isotype: IgG1

Clone: DREG56

Description: CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cellsurface of leukocytes and mediates their homing to inflammatory sites and peripheral lymphnodes by enabling rolling along the venular wall. CD62L is also involved inactivation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however,counteracts neutrophil rolling. CD62L has also signaling roles including enhance ofchemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1(P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to helpdistinguish naive T cells from effector/memory T cells.

Specificity: The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cellsurface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells,that interacts with specific carbohydrates exposed on activated endothelial cells.HLDA V, WS Code S056

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only