Selectin L (SELL) antibody

Antigen: Selectin L (SELL)

Synonyms: TQ1, LAM1, LNHR, LSEL, CD62L, LAM-1, LYAM1, Leu-8, PLNHR, hLHRc, LECAM1, Lyam-1, Lnhr, Ly-22, Lyam1, Ly-m22, LECAM-1, AI528707, L-selectin

Clonality: Monoclonal (DREG56)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Catalog no.: ABIN457418

Additional Information

Alternative name: CD62L

Immunogen: PMA-activated human peripheral blood leukocytes

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: DREG56

Description: CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cellsurface of leukocytes and mediates their homing to inflammatory sites and peripheral lymphnodes by enabling rolling along the venular wall. CD62L is also involved inactivation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however,counteracts neutrophil rolling. CD62L has also signaling roles including enhance ofchemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1(P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to helpdistinguish naive T cells from effector/memory T cells.

Specificity: The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cellsurface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells,that interacts with specific carbohydrates exposed on activated endothelial cells.HLDA V, WS Code S056

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 2 µg/ml. Application note: non-reducing conditionsImmunohistochemistry (frozen sections). Functional ApplicationParticularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymphnode HEV

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only