IgA (alpha chain) antibody

Antigen: IgA (alpha chain)

Clonality: Polyclonal

Host: Pig

Reactivity: Goat

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Catalog no.: ABIN457730

Additional Information

Characteristics: Precipitating polyclonal swine antiserum to goat IgA

Alternative name: IgA

Immunogen: Highly purified secretory IgA isolated from pooled goat milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Description: The reactivity of the antiserum is restricted to the Fc part of the IgA molecule as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal goat serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. Cross-reactivity The antiserum does not cross-react with any other component of the goat Ig system or any other plasma protein. Inter-species cross-reactivity is a normal feature of antibodies to immuno-globulins, since Ig of different species frequently share antigenic determinants.

Specificity: The antiserum does not cross-react with any other component of the goat Ig system or any other plasma protein. Inter-species cross-reactivity is a normal feature of antibodies to immuno-globulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in goat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of goat IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal swine serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal goat serum in agar-block immunodiffusion titrati

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum Preservative: No preservative added.

Storage: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Restrictions: For Research Use only