IgG (H&L) antibody

Antigen: IgG (H&L)

Clonality: Polyclonal

Host: Pig

Reactivity: Horse (Equine)

Application: Immunodiffusion (ID), ELISA, Immunoelectrophoresis (IEP), Nephelometry (Neph), Radial Immunodiffusion (RID)

Catalog no.: ABIN457812

Additional Information

Characteristics: Precipitating polyclonal swine antiserum to horse IgG, heavy and light chains

Alternative name: IgG (Heavy and Light Chains)

Immunogen: Purified normal IgG isolated from pooled horse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Description: The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses IgG1 and IgG2. Reactivity with other subclasses of horse IgG has not been tested. The antiserum reacts with both Fc and Fab portions of polyclonal IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against horse serum usually a single characteristic precipitin line is observed, representing primarily IgG2. If the level of IgG1, also known as IgG(T), in the serum sample approaches the level of IgG2, it may become visible as a second precipitin line. It does not react with any non-Ig protein in horse serum, as tested by immunoelectrophoresis and double radial immuno-diffusion. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants.

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Application Details

Application Notes: In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal horse serum, to prepare an immunoadsorbent for the production of Ig-free horse serum, plasma or non-Ig preparations. It has not been tested for use in nephelometry, ELISA or immunohistology, but this does not exclude such use if proper controls are included. Adsorption: No adsorption required.

Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled swine serum. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal horse serum in agar block titration.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum Preservative: No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Storage: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Restrictions: For Research Use only