IgA, Isotype Specific antibody

Antigen: IgA, Isotype Specific

Clonality: Monoclonal (NI 69 (A89-034) and NI 184 (A89-035))

Host: Mouse

Reactivity: Human

Application: Haemagglutination (Haema), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Catalog no.: ABIN457921

Additional Information

Characteristics: Monoclonal mouse antiserum to human IgA, isotype specific

Immunogen: Highly purified monoclonal IgA isolated from pooled human serum

Isotype: IgG1 kappa

Clone: NI 69 (A89-034) and NI 184 (A89-035)

Description: The reactivity of the antiserum is restricted to determinants on the C H 2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148).

Specificity: The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.

Application Details

Application Notes: To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Concentration: IgG concentration is 1 mg/ml. No foreign proteins added.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole ascites

Storage: Lyophilized at +4ºC (10 years), reconstituted at or below –20°C (3-5 years), reconstituted at +4°C at least (7 days). The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized ascites by adding 0.5 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day.

Restrictions: For Research Use only