Inter alpha Trypsin Inhibitor antibody

Catalog No. ABIN458167

This Sheep Polyclonal antibody specifically detects Inter alpha Trypsin Inhibitor in IEP. It exhibits reactivity toward Human.

Quick Overview for Inter alpha Trypsin Inhibitor antibody (ABIN458167)

Target: Inter alpha Trypsin Inhibitor

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunoelectrophoresis (IEP)

Product Details

Specificity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal sheep antiserum to human inter alpha trypsin inhibitor

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Inter alpha trypsin inhibitor is a proteinase inhibitor with a molecular weight of 160,000. Its mean concentration in human plasma is about 400 µ g/ml. It has been isolated as a homogenous protein for use in the antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single and double radial immuno-diffusion (Mancini, Ouchterlony) to identify the presence of inter alpha trypsin inhibitor or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: IgG fraction 13 mg/ml in normal sheep serum diluted 1/2 with PBS. No foreign proteins added. Antibody titre: Precipitin titre not less than 1:64 when tested against normal human plasma in agar-block immunodiffusion titration.

Buffer: Delipidated, heat inactivated, lyophilized, stable IgG fraction in ½ diluted normal serum.

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconsti tute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.

Target Details

Target: Inter alpha Trypsin Inhibitor

Background: The reactivity of the antiserum is restricted to inter alpha trypsin inhibitor. In immunoelectro-phoresis and radial immunodiffusion, using various antiserum concentrations against human plasma a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen