Rabbit anti-Rabbit IgG, IgA,IgM (H&L) Antibody (HRP)

Antigen: IgG, IgA,IgM (H&L)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rabbit

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Catalog no.: ABIN458613

Additional Information

Characteristics: Horseradish peroxidase–conjugated IgG fraction of polyclonal monkey antiserum to rabbit immunoglobulins IgG, IgA and IgM, heavy and light chains

Alternative name: IgG, IgA, IgM (Heavy and Light Chains)

Immunogen: Purified immunoglobulin IgG, IgA and IgM fractions isolated from pooled rhesus monkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Description: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in monkey serum, as tested by immuno-electrophoresis and double radial immunodiffusion. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants.

Specificity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Application Details

Application Notes: In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in monkey serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1: 500, in ELISA and comparable non-precipitating antibody-binding assays between 1:2,000 and 1:8,000. Adsorption: No adsorption required.

Concentration: IgG protein concentration 10 mg/ml. Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).

Purification: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Buffer: Peroxidase-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). Preservative: No preservative added, as it may interfere with the antibody activity.

Storage: Lyophilised at +4°C (10 years), reconstituted at or below -20°C (3-5 years), reconstituted at +4°C at least 7 days. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Restrictions: For Research Use only