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Goat anti-Rat IgG2ab, Subclass Specific Antibody (TRITC) - Preadsorbed

ICC, IHC Polyclonal IgG TRITC
Catalog No. ABIN458698
  • Target See all IgG2ab products
    IgG2ab (IgG2ab, Subclass Specific)
    Reactivity
    Rat
    Host
    • 7
    • 1
    Goat
    Clonality
    • 8
    Polyclonal
    Conjugate
    • 3
    • 2
    • 1
    • 1
    • 1
    TRITC
    Application
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    Immunocytochemistry (ICC), Immunohistochemistry (IHC)
    Specificity
    Specificity of this antiserum has not been tested in detail, however in double radial immunodiffusion a weak reaction with mouse has been observed.
    Purification
    Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
    Immunogen
    Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Application Notes
    In immunocytochemical and immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80, depending on the method used.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Concentration
    10 mg/mL
    Buffer
    TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Preservative
    Without preservative
    Handling Advice
    Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    Storage
    4 °C/-20 °C
    Storage Comment
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target
    IgG2ab (IgG2ab, Subclass Specific)
    Alternative Name
    IgG2ab (IgG2ab Products)
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