F13A1 antibody

Catalog No. ABIN461969

The Sheep Polyclonal anti-F13A1 antibody is suitable to detect F13A1 in samples from Human. It has been validated for IHC, ELISA, IHC (p) and IEP.

Quick Overview for F13A1 antibody (ABIN461969)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This F13A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoelectrophoresis (IEP)

Product Details anti-F13A1 Antibody

Specificity: A single precipitin line was seen against normal plasma showing identity to that obtained with purified Factor XIII. No band was observed with Factor XIII DP.

Purification: Ammonium sulfate precipitation

Immunogen: Human Factor XIII (A subunit only) purified from plasma.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IE, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.4, 0.15 M sodium chloride, 50 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: F13A1 / Factor XIIIa

Background: Name/Gene ID: F13A1

Synonyms: F13A1, Coagulation factor XIIIa, Factor XIIIa, Fibrinoligase, FSF, A subunit, Transglutaminase. plasma, Transglutaminase A chain, F13A, TGASE

Gene ID: 2162

UniProt: P00488