MADCAM1 antibody

Catalog No. ABIN2625029

The Mouse Monoclonal anti-MADCAM1 antibody (Clone 314G8) (ABIN2625029) specifically detects MADCAM1 in WB, ELISA, FACS, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for MADCAM1 antibody (ABIN2625029)

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MADCAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 314G8

Product Details anti-MADCAM1 Antibody

Specificity: Recognizes human mucosal addressin cell adhesion molecule 1 (MAdCAM-1), a 60kD cell surface protein that is involved in lymphocyte trafficking. MAdCAM-1 is expressed on high endothelial venules of Peyers patches and mesenteric lymph nodes. MAdCAM-1 expression has also been reported in the gut lamina propria but clone 314G8 does not recognize MAdCAM-1 in these tissues. Clone Reacts with the ligand-binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognized by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM-1 with alpha 4 beta 7.

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: Recombinant soluble MAdCAM-1-fc fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:10 - 1:100), IHC, IHC-P (10 μg/mL), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin embedded sections prior to staining. This product does not require antigen retrieval prior to staining of paraffin sections. Western Blot: LS-C44686 detects a band of approximately 60kD. Reducing conditions required. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.

Target Details for MADCAM1

Target: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))

Alternative Name: MADCAM1 / MAdCAM-1

Background: Name/Gene ID: MADCAM1

Synonyms: MADCAM1, HMAdCAM-1, MACAM1, MAdCAM-1

Gene ID: 8174

UniProt: Q13477