F13A1 antibody

Catalog No. ABIN462397

This Goat Polyclonal antibody specifically detects F13A1 in IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for F13A1 antibody (ABIN462397)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This F13A1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-F13A1 Antibody

Specificity: Human F13A1 / Factor XIIIa.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset, Panda (93%) Mouse, Rat, Hamster, Bat, Bovine, Dog, Horse, Pig (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TSVHLFKERWDTNK from an internal region of human F13A1 / Factor XIIIa (NP_000120.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset, Panda (93%), Mouse, Rat, Hamster, Bat, Bovine, Dog, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-6 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: F13A1 / Factor XIIIa

Background: Name/Gene ID: F13A1

Synonyms: F13A1, Coagulation factor XIIIa, Factor XIIIa, Fibrinoligase, FSF, A subunit, Transglutaminase. plasma, Transglutaminase A chain, F13A, TGASE

Gene ID: 2162

NCBI Accession: NP_000120

UniProt: P00488