WT1 antibody (N-Term)

Catalog No. ABIN6740526

The Rabbit Polyclonal anti-WT1 antibody (ABIN6740526) specifically detects WT1 in WB. The antibody is reactive with Human samples.

Quick Overview for WT1 antibody (N-Term) (ABIN6740526)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: N-Term

Specificity: Human WT1

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human WT1 (NP_000369). Percent identity by BLAST analysis: Human (100%), Gorilla, Marmoset (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 / Wilms Tumor 1

Background: Name/Gene ID: WT1

Synonyms: WT1, GUD, EWS-WT1, Amino-terminal domain of EWS, WAGR, WIT-2, Wilms tumor protein, AWT1, Wilms tumor 1, WT33, NPHS4

Gene ID: 7490

NCBI Accession: NP_000369

UniProt: P19544

Pathways: Tube Formation