RANKL antibody (Biotin)

Catalog No. ABIN465061

The Biotin-conjugated Rabbit Polyclonal anti-RANKL antibody (ABIN465061) specifically detects RANKL in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for RANKL antibody (Biotin) (ABIN465061)

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANKL antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-RANKL Antibody (Biotin)

Specificity: Human sRANK Ligand

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human sRANK Ligand.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hsRANKL by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. To detect hsRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sRANKL (LS-B7418) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for RANKL

Target: RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))

Alternative Name: TNFSF11 / RANKL / TRANCE

Background: Name/Gene ID: TNFSF11
Family: TNF

Synonyms: TNFSF11, CD254, CD254 antigen, HRANKL2, OPTB2, TRANCE, ODF, OPGL, SOdf, Osteoprotegerin ligand, RANKL

Gene ID: 8600

UniProt: O14788

Pathways: NF-kappaB Signaling