CCL25 antibody

Catalog No. ABIN465067

The Rabbit Polyclonal anti-CCL25 antibody (ABIN465067) specifically detects CCL25 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for CCL25 antibody (ABIN465067)

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL25 Antibody

Specificity: Human TECK

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TECK.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (10 - 15 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal small bowel. The recommended concentration is 0.5 μg/mL-1.0 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hTECK (100 ng/mL), a concentration of 10-15 μg/mL of this antibody is required. ELISA: To detect hTECK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human TECK (LS-C104509) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTECK is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL25

Target: CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))

Alternative Name: TECK / CCL25

Background: Name/Gene ID: CCL25
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL25, C-C motif chemokine 25, Ck beta-15, TECK, Thymus-expressed chemokine, Small-inducible cytokine A25, TECKvar, Chemokine TECK, Ckb15, SCYA25, Thymus expressed chemokine

Gene ID: 6370

UniProt: O15444