TNFRSF1A antibody

Catalog No. ABIN465074

The Rabbit Polyclonal anti-TNFRSF1A antibody is suitable to detect TNFRSF1A in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for TNFRSF1A antibody (ABIN465074)

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-TNFRSF1A Antibody

Specificity: Human sTNF Receptor-I

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human sTNF Receptor I.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.9 - 1.1 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hsTNF-receptor (0.3 μg/mL), a concentration of 0.9-1.1 μg/mL of this antibody is required. ELISA: To detect hsTNF-receptor by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor (LS-C104403) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor. Western Blot: To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFRSF1A

Target: TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))

Alternative Name: TNFRSF1A / TNFR1

Background: Name/Gene ID: TNFRSF1A
Family: TNF Receptor

Synonyms: TNFRSF1A, CD120a, CD120a antigen, MS5, p60, TBP1, TNF-R55, TNFAR, TNFR1, p55-R, TNF-R, TNF-R-I, TNF-R1, TNF-RI, Tnfr, TNFR-I, TNFR1-d2, TNFR60, FPF, p55, Tbpi, TNFR55

Gene ID: 7132

UniProt: P19438

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway