CCL1 antibody

Catalog No. ABIN465124

This Rabbit Polyclonal antibody specifically detects CCL1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL1 antibody (ABIN465124)

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL1 Antibody

Specificity: Human I-309

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human I-309.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal pancreas. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hI-309 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human I-309 (LS-C104348) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hI-309. Western Blot: To detect human I-309 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human I-309 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Alternative Name: I-309 / CCL1

Background: Name/Gene ID: CCL1
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL1, C-C motif chemokine 1, I-309, p500, Inflammatory cytokine I-309, TCA3, SISe, Small-inducible cytokine A1, Chemokine (C-C motif) ligand 1, SCYA1

Gene ID: 6346

UniProt: P22362