CCL14 antibody

Catalog No. ABIN465136

This Rabbit Polyclonal antibody specifically detects CCL14 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CCL14 antibody (ABIN465136)

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCL14 Antibody

Specificity: Human HCC-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human HCC-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Proteinase K antigen retrieval is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hHCC-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human HCC-1 (LS-C104432) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHCC-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL14

Target: CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))

Alternative Name: Hcc1 / CCL14

Background: Name/Gene ID: CCL14
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL14, CC-3, C-C motif chemokine 14, Chemokine cc3, Chemokine CC-1/CC-3, Chemokine cc1, Chemokine CC-3, HCC-1(1-74), Hcc3, HCC-1, HCC-3, MCIF, NCC-2, SCYA14, Small-inducible cytokine A14, SY14, CC-1, CKB1, HCC1, Hemofiltrate CC chemokine 1, NCC2, New CC chemokine 2

Gene ID: 6358

UniProt: Q16627