CCL13 antibody

Catalog No. ABIN465170

The Mouse Monoclonal anti-CCL13 antibody is suitable to detect CCL13 in samples from Human. It has been validated for WB, ELISA and Neut.

Quick Overview for CCL13 antibody (ABIN465170)

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCL13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL13 Antibody

Specificity: Human MCP-4

Purification: Protein A purified

Immunogen: E.coli derived recombinant Human MCP-4.

Type of Immunogen: Cell extract

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 4 μg/mL), Neut (1 - 5 μg/mL), WB (0.2 - 0.4 μg/mL)

Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human MCP-4 (100 ng/mL), a concentration of 0.8-1 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human MCP-4 when used with biotinylated antigen affinity purified anti-human MCP-4 (LS-C104448) as the detection antibody at a concentration of approximately 0.5-1 μg/mL. Western Blot: To detect hMCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-4 is 2-4 ng/lane, under non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL13

Target: CCL13 (Chemokine (C-C Motif) Ligand 13 (CCL13))

Alternative Name: CCL13 / MCP-4

Background: Name/Gene ID: CCL13
Family: Intercrine

Synonyms: CCL13, C-C motif chemokine 13, CKb10, MCP-4, MCP4, Monocyte chemotactic protein 4, New CC chemokine 1, Small-inducible cytokine A13, NCC1, CK-beta-10, NCC-1, SCYA13

Gene ID: 6357

UniProt: Q99616

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, The Global Phosphorylation Landscape of SARS-CoV-2 Infection