TNFSF13 antibody (Biotin)

Catalog No. ABIN465171

The Biotin-conjugated Goat Polyclonal anti-TNFSF13 antibody (ABIN465171) specifically detects TNFSF13 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for TNFSF13 antibody (Biotin) (ABIN465171)

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TNFSF13 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-TNFSF13 Antibody (Biotin)

Specificity: Human sFas Ligand

Purification: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human sFas Ligand.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect hFasL/Apo1L by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hFasL/Apo1L. To detect hFasL/Apo1L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human FasL/Apo1L (LS-C104463) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hFasL/Apo1L. Western Blot: To detect hFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1L is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: TNFSF13 / APRIL

Background: Name/Gene ID: TNFSF13
Family: TNF

Synonyms: TNFSF13, APRIL, CD256, CD256 antigen, TALL-2, TALL2, ZTNF2, TNF-related death ligand 1, TRDL-1, UNQ383/PRO715

Gene ID: 8741

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response